Light microscopy in biology

Since the first edition of Light Microscopy in Biology: A Practical Approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes new material on topics from the first edition as well as a number of completely new approaches. It begins with an explanation of the basic techniques and goes on to describe current methods in chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. New topics include confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. It also includes newly added chapters devoted to reflection contrast microscopy and histomorphometry. This edition will prove an invaluable resource for postgraduate and postdoctoral researchers in biomedicine and cell biology--both those experienced with the techniques and newcomers to the field.